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ANALYSES OF GENETIC VARIABILITY IN LENTINULA EDODES THROUGH MYCELIA RESPONSES TO DIFFERENT ABIOTIC CONDITIONS AND RAPD MOLECULAR MARKERS BJM
Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto.
The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edible mushroom; RAPD; Mycelial growth.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002
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Antagonism of Trichoderma spp. strains against pea ( Pisum sativum L.) Fusarium wilt caused by Fusarium oxysporum f. sp. pisi. Acta Agron. (Palmira)
Checa Coral,Oscar Eduardo; Toro Criollo,Maribel; Descanse Vallejo,Jesica.
Abstract The antagonistic effectiveness of native strains of Trichoderma spp. on Fusarium oxysporum f. sp. pisi. in vitro, greenhouse and field conditions, were evaluated. in vitro conditions, the antagonistic capacity of 12 strains of Trichoderma spp., C2, C7, C12 and C21 strains, exhibited a better behavior measured by the following variables: inhibition halo and mycelial growth. In greenhouse conditions, the four strains, which showed the best in vitro antagonistic behavior, were evaluated using a DIA experimental design with factorial arrangement for three factors, which corresponded to strain, concentration and dose. The results of this evaluation, showed that C12 and C21strains at doses of 20 mL, and at concentrations of 108 and 106 conidia.mL-1,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonistic capacity; Inhibition halo; Inoculum; In vitro; Field conditions; Greenhouse; Mycelial growth.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000300442
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Caracterização morfofisiológica e identificação molecular de isolados de Guignardia citricarpa, agente patogênico da mancha preta dos citros - DOI: 10.4025/actasciagron.v30i5.5962 Agronomy
Caixeta, Marilda Pereira; UEM; Corazza, Maria Júlia; UEM; Oliveira, Ricardo Ribeiro de; UEM; Zanutto, Carlos Alexandre; UEM; Nunes, William Mário de Carvalho; UEM; Vida, João Batista; UEM.
O presente trabalho teve como objetivo identificar 11 isolados de Guignardia citricarpa, agente causal da mancha preta dos citros (MPC), obtidos de frutas cítricas sintomáticas de diferentes regiões geográficas, por meio da PCR e caracterização morfofisiológica das estruturas propagativas, esporulação e crescimento micelial em diferentes meios de cultura, temperaturas e regimes de luz, nas condições de laboratório. Pelo teste de PCR, todos os isolados foram identificados como o patógeno G. citricarpa. Os isolados caracterizados foram submetidos às temperaturas de 20, 25 e 30ºC, em regime de luz contínua, escuro contínuo e fotoperíodos de 12 horas, durante 24 dias. Utilizaram-se os meios de cultura aveia-ágar (AA), batata-dextrose-ágar (BDA) e...
Palavras-chave: 5.01.02.00-1 Fitossanidade G. citricarpa; PCR; Esporulação; Crescimento micelial; Temperatura; Meio de cultura; Fotoperíodo. G. citricarpa; PCR; Sporing; Mycelial growth; Temperature; Mean of culture; Photoperiod.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5962
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Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method - DOI: 10.4025/actasciagron.v32i1.5086 Agronomy
Zaghi Junior, Lienine Luiz; UNIPAR; Linde, Giani Andrea; UNIPAR; Colauto, Nelson Barros; Unipar.
Cultivation techniques for Agaricus brasiliensis (composting method) and substratum formulations are the same ones used for Agaricus bisporus. Most of the carbon-to-nitrogen (C:N) ratios reported for A. brasiliensis are similar to those used for A. bisporus on the composting method and there are few studies about the variation of C:N ratios for A. brasilienses on the axenic method. The objective of this study was to verify the mycelial growth of A. brasiliensis on different C:N ratios using regional by-products as substrate formulation on the axenic method. Studied C:N ratios of substrate (mixture of soybean and cassava fibers) ranged from 11:1 to 248:1, with nitrogen content ranging from 4.25 to 0.20%, respectively. It was concluded that substrate with...
Palavras-chave: 5.01.00.00-9 Agronomia Agaricus blazei; Mycelial growth; Substrate; Cultivation Microbiologia Agrícola.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5086
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Comparison of radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa forel in two culture media BJM
Miyashira,C.H; Tanigushi,D.G; Gugliotta,A.M; Santos,D.Y.A.C.
In vitro culture of the mutualistic fungus of leaf-cutting ants is troublesome due to its low growth rate, which leads to storage problems and contaminants accumulation. This paper aims at comparing the radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa Forel in two different culture media (Pagnocca B and MEA LP). Although total MEA LP radial growth was greater all along the bioassay, no significant difference was detected between growth efficiencies of the two media. Previous evidences of low growth rate for this fungus were confirmed. Since these data cannot point greater efficiency of one culture medium over the other, MEA LP medium is indicated for in vitro studies with this mutualistic fungus due its simpler composition and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leaf-cutting ants; Mutualistic fungus growth; Mycelial growth; Leucoagaricus; Atta sexdens.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200035
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Cultivation of Flammulina velutipesmushroom using various agro-residues as a fruiting substrate PAB
Harith,Nooraishah; Abdullah,Noorlidah; Sabaratnam,Vikineswary.
The objective of this work was to evaluate the feasibility of different agro-residues as a carbon source in the fruiting substrates of Flammulina velutipesmushroom and the effect of supplementation with the nitrogen sources spent brewer's yeast and rice bran. The following fruiting substrates were evaluated: rubber wood sawdust (SD), paddy straw (PS), palm empty fruit bunches (EFB), and palm-pressed fiber (PPF). Cultivation was done on each agro-residue, based on formulations consisting of two substrates at the ratios of 3:1, 1:1, and 1:3. Mycelial growth rate and basidiocarp yield were evaluated. The best fruiting substrates were PS+EFB (25:75), PS+PPF (50:50), and PPF (100), with biological efficiency of 185.09±36.98, 150.89±50.35, and 129.06±14.51%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological efficiency; Golden needle mushroom; Mycelial growth; Oil palm waste; Paddy straw.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014000300181
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Difference between isolates from brown eye spot and black spot lesions in coffee plants PAB
Botelho,Deila Magna dos Santos; Resende,Mário Lucio Vilela de; Rezende,Juliana Costa de; Ribeiro Júnior,Pedro Martins; Andrade,Camila Cristina Lage de; Alves,Eduardo; Amaral,Douglas Carvalho.
Abstract: The objective of this work was to evaluate the difference between the isolates from two cercospora leaf spot symptoms (brown eye spot and black spot) in relation to conidial morphology, mycelial growth, cercosporin production, and reproduction of symptoms in coffee leaves collected in three municipalities in the state of Minas Gerais, Brazil. There was a difference between the isolates for the studied variables, but without any relation to the type of symptom. The differences found were not associated with the origin of the isolates. The symptoms characterized as black spot were associated with different host conditions during the infection process.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cercospora coffeicola; Coffea arabica; Cercosporin production; Conidial morphology; Mycelial growth; Reproduction of symptoms.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102302
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Effect of surfactants and temperature on germination and vegetative growth of Beauveria bassiana BJM
Mwamburi,Lizzy A.; Laing,Mark D.; Miller,Ray M..
Three non-ionic surfactants: Tween20, Tween80 and Breakthru® were screened for their effects on spore germination and mycelial growth rates and for their influence on three isolates of Beauveria bassianaspore germination at various temperatures. Tween20 and Tween80 were compatible with all the B. bassiana isolates in the germination studies, but inhibited germination at higher surfactant concentrations, irrespective of the conidial concentrations. Breakthru® had an inhibitory effect on germination even at the lowest concentration of 0.1% on all the B. bassiana isolates. The effects of the surfactants on spore germination did not correspond with their effects on colony growth. Conidial viability within the same formulation declined significantly with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beauveria bassiana; Surfactant; Temperature; Conidia germination; Mycelial growth.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100067
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Essential oils in the control of Colletotrichum gloeosporioides f. Sp. cepae in onion seeds Rev. Ciênc. Agron.
Lozada,Maria Isabel Ordonez; Silva,Patricia Pereira da; Pereira,Ricardo Borges; Nascimento,Warley Marcos.
ABSTRACT Anthracnose, one of the major diseases in the onion, is caused by the fungus Colletotrichum gloeosporioides f. sp. cepae. The main strategy for controlling this fungus is by the use of fungicide, with this technique being used on the majority of crops. Currently, methods of disease control are being studied that are less aggressive to the environment, with the use of essential oils proving to be an alternative. As such, the aim of this study was to evaluate the direct effect of the essential oils of basil (Ocimum basilicum), lemongrass (Cymbopogon citratus (DC) Stapf.), sage (Salvia officinalis), thyme (Thymus vulgaris) and citronella (Cymbopogon winterianus) as an alternative in the control of the fungus C. gloeosporioides f. sp. cepae, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Physiological quality; Conidial germination; Mycelial growth.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000300510
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Germination and mycelial growth of Bipolaris euphorbiae Muchovej & Carvalho as influenced by herbicides and surfactants BJM
Nemoto,Marta Cecília Mallasen; Nahas,Ely; Pitelli,Robinson Antonio; Coelho,Lisias.
Bipolaris euphorbiae Muchovej & Carvalho can be combined with herbicide in order to control a large spectrum of weed species, being a strong candidate for the biocontrol of Euphorbia heterophylla L. (milk weed). The fungus release can be combined with herbicide in order to control a broader spectrum of weed species. Thus, laboratory experiments were set up to study the feasibility of using tank mixes of B. euphorbiae spores with herbicides or surfactants recommended for soybean. Mycelial growth and conidia germination were evaluated in PDA medium enriched with the herbicides oxasulfuron (80 g/ha), glyphosate (4 L/ha), bentazon (1.5 L/ha), fomesafen (1 L/ha), chlorimuron-ethyl (80 g/ha), lactofen (1 L/ha) and imazetaphyr (1 L/ha), and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris euphorbiae; Mycelial growth; Conidia germination; Herbicides; Surfactants.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400015
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Growth and antibacterial activity of Lentinula edodes in liquid media supplemented with agricultural wastes Electron. J. Biotechnol.
Hiroko Hassegawa,Regina; Megumi Kasuya,Maria Catarina; Dantas Vanetti,Maria Cristina.
Antibacterial activity of Lentinula edodes against Bacillus subtilis was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L. edodes dry mycelial biomass in liquid culture with 0.5% added rice bran was 3.2 mg/ml, after growth for 30 days at 25ºC without shaking, and 4.3 mg/ml under orbital shaking (150 rpm). However, antibacterial activity, detected between 20 and 24 days of incubation of stationary cultures, was absent in filtrates of aerated cultures. Temperatures of 20-25ºC enhanced both growth and antibacterial activity. Optimum pH for L. edodes mycelial growth...
Tipo: Journal article Palavras-chave: Bacterial inhibition; Bioconversion; Mycelial growth; Mushroom; Shiitake.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200011
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Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes Scientia Agricola
Onofre,Sideney Becker; Miniuk,Cindia Mara; Barros,Neiva Monteiro de; Azevedo,João Lúcio.
Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used....
Tipo: Info:eu-repo/semantics/report Palavras-chave: Metarhizium flavoviride; Mycelial growth; Culture media; Lighting regimes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300026
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Growth of symbiont fungi of some higher attine ants in mineral medium Ciência Rural
Loeck,Alci Enimar; Pierobom,Carlos Roberto; Gusmão,Luciana Guerra de; Afonso,Ana Paula.
Bioassays were conducted to verify the possibility of culturing the symbiont fungus of some higher attine in mineral medium and finding out the optimum pH value for their satisfactory mycelial growth. Three organic media and one mineral medium were inoculated with isolates from Atta sexdens piriventris and Acromyrmex heyeri. In mineral medium different values of pH (4.0, 5.0, 6.0 and 7.0) were tested with isolates from A. laevigata and A. laticeps. The behavior of isolates (colony diameter) was different in the mineral medium. However, even the one which grew the least of all provided enough mycelial for RAPD analysis. The best range of pH for fungal growth in mineral medium was between 4.0 and 5.0
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leaf-cutting ants; Symbiont fungus; Mycelial growth.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100012
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Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region BJM
Guadarrama-Mendoza,P.C.; Valencia del Toro,G.; Ramírez-Carrillo,R.; Robles-Martínez,F.; Yáñez-Fernández,J.; Garín-Aguilar,M.E.; Hernández,C.G.; Bravo-Villa,G..
Two native Pleurotus spp. strains (white LB-050 and pale pink LB-051) were isolated from rotten tree trunks of cazahuate (Ipomoea murucoides) from the Mexican Mixtec Region. Both strains were chemically dedikaryotized to obtain their symmetrical monokaryotic components (neohaplonts). This was achieved employing homogenization time periods from 60 to 65 s, and 3 day incubation at 28 °C in a peptone-glucose solution (PGS). Pairing of compatible neohaplonts resulted in 56 hybrid strains which were classified into the four following hybrid types: (R1-n xB1-n, R1-n xB2-1, R2-n xB1-n and R2-n xB2-1). The mycelial growth of Pleurotus spp. monokaryotic and dikaryotic strains showed differences in texture (cottony or floccose), growth (scarce, regular or abundant),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pleurotus; Mycelial growth; Mycelial morphology; Hybrid strains; Monokaryotization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300016
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Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota) Acta Amazonica
SANTANA,Marcos Diones Ferreira; VARGAS-ISLA,Ruby; NOGUEIRA,Janaina da Costa; ACCIOLY,Thiago; SILVA,Bianca Denise Barbosa da; COUCEIRO,Sheyla Regina Marques; BASEIA,Iuri Goulart; ISHIKAWA,Noemia Kazue.
ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Earthstars; Gasteroid fungus; Geastrum lloydianum; Geastrum subiculosum; Mycelial growth.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100061
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Obtención de híbridos mejorados de shii-take (lentinula edodes) para fortalecer la tranferencia de su tecnología. Colegio de Postgraduados
Galván Pallach, Fernando.
Esta investigación consistió en formar híbridos entre diferentes cepas de Lentinula edodes que presentaran equivalentes o mejores resultados y características que sus cepas progenitoras, Se evaluaron 16 cepas de Lentinula edodes. Para después realizar cruzas DI-MON entre las cepas de Lentinula edodes y los monospóricos de la cepa CP-7, y cruzas MON-MON entre los monospóricos de la cepa CP-7 y CP-188. Para el crecimiento micelial en masters, los híbridos DI-MON [97] X [7]32, [189] X [7]11 y [95] X [7]11, fueron los más precoces. Para el crecimiento micelial en bolsas, las cepas CP-172, CP-174, CP-188*, CP-97, CP-189, CP-7*, CP-163 y el híbrido MON-MON [7]32 X [188]29, fueron los mas precoces. Para la formación de costra café, el híbrido MON-MON [7]32 X...
Tipo: Tesis Palavras-chave: Lentinula edodes; Cepa; Híbrido; Crecimiento micelial; Fructificación; Maestría; EDAR; Estrategías para el Desarrollo Agrícola Regional; Strain; Hybrid; Mycelial growth; Fruiting.
Ano: 2008 URL: http://hdl.handle.net/10521/1452
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Optimization of nutritional requirements for mycelial growth and sporulation of entomogenous fungus Aschersonia aleyrodis Webber BJM
Zhu,Yanping; Pan,Jieru; Qiu,Junzhi; Guan,Xiong.
The objective of the present study was to investigate the optimal nutritional requirements for mycelial growth and sporulation of entomopathogenic fungus Aschersonia aleyrodis Webber by orthogonal layout methods. Herein the order of effects of nutrient components on mycelial growth was tryptone > Ca2+ > soluble starch > folacin, corresponding to the following optimal concentrations: 1.58% Soluble Starch, 3.16% Tryptone, 0.2 mmol l-1 Ca2+ and 0.005% Folacin. The optimal concentration of each factors for sporulation was 1.16% lactose, 0.394% tryptone, 0.4 mmol l-1 Fe2+ and 0.00125% V B1, and the effects of medium components on sporulation were found to be in the order lactose > V B1 > Fe2+ > tryptone. Under the optimal culture conditions,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomogenous fungus; Orthogonal matrix method; Mycelial growth; Sporulation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400032
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Spore production in Paecilomyces lilacinus (Thom.) samson strains on agro-industrial residues BJM
Robl,Diogo; Sung,Letizia B.; Novakovich,João Henrique; Marangoni,Paulo R. D.; Zawadneak,Maria Aparecida C.; Dalzoto,Patricia R.; Gabardo,Juarez; Pimentel,Ida Chapaval.
Paecilomyces lilacinus has potential for pests control. We aimed to analyze mycelial growth and spore production in P. lilacinus strains in several agro-industrial residues and commercial media. This study suggests alternative nutrient sources for fungi production and that the biotechnological potential of agro-industrial refuses could be employed in byproducts development.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Mycelial growth; Nematophagous fungus.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200016
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